IDFP basic Characters:
IDFP (615250-02-7) cycle
Chemical Names:ISOPROPYL DODECYLFLUOROPHOSPHONATE
IDFP;ISOPROPYL DODECYLFLUOROPHOSPHONATE;P-DODECYL-1-METHYLETHYL ESTER-PHOSPHONOFLUORIDIC ACID
IDFP (615250-02-7) Usage
IDFP is an organophosphorus compound related to the nerve agent sarin. Like sarin, IDFP is an irreversible inhibitor for a number of different enzymes that normally serve to break down neurotransmitters, however the long alkyl chain of IDFP makes it dramatically weaker as an inhibitor of acetylcholinesterase (AChE), with an IC50 of only 6300nM, while it is a potent inhibitor of two enzymes monoacylglycerol lipase (MAGL), the primary enzyme responsible for degrading the endocannabinoid 2-arachidonoylglycerol (2-AG), and fatty acid amide hydrolase (FAAH), the primary enzyme that degrades the other main endocannabinoid anandamide. The IC50 of IDFP is 0.8nM at MAGL, and 3.0nM at FAAH. Inhibition of these two enzymes causes markedly increased levels of both anandamide and 2-AG in the brain, resulting inincreased cannabinoid signalling and typical cannabinoid behavioral effects in animal studies, while its lack of potency at AChE means that no cholinergic symptoms are produced. Despite its similar chemical structure to the banned nerve agents, the long alkyl chain of IDFP causes it to fall outside the definition of “toxic chemicals” under the Chemical Weapons Convention, and since it also does not exhibit the potent AChE inhibition of related organophosphorus compounds, IDFP is not subject to the same stringent legal controls.
What is the dosage of IDFP
For long term storage,we suggest that IDFP be stored as supplied at -20℃. It should be stable for at least one year.
IDFP is supplied as solution in methyl acetate. To change the solvent,simply evaporate the methyl acetate under a gentle stream of nitrogenand immediately add the solvent of choice.Solvents such as ethanol,DMSO,and dimethyl formamide purged with an inert gas can be used. The solubility of IDFP in these solvents is approximately 10mg/ml.
If aqueous stock solutions are required for biological experiments,they can best be prepared by diluting the organic solvent into aqueous buffers or isotonic saline.Ensure that the residual amount of organicsolvent is insignificant,since organic solvents may have physiological effects at low concentrations.We do not recommend storingthe aqueous solution for more than one day.
How IDFP works
IDFP is an irreversible inhibitor for a number of different enzymes that normally serve to break down neurotransmitters, however the long alkyl chain of IDFP makes itdramatically weaker as an inhibitor of acetylcholinesterase (AChE), while it is a potent inhibitor of two enzymes monoacylglycerol lipase (MAGL), the primary enzyme responsible for degrading the endocannabinoid 2-arachidonoylglycerol (2-AG), and fatty acid amide hydrolase (FAAH), the primary enzyme that degrades the other mainendocannabinoid anandamide. An organophosphorus compound that dually inhibits MAGL and FAAH with IC50 values of 0.8 and 3 nM, respectively.1 At 10 mg/kg, IDFP elevates brain levels of 2-AG and AEA more than 10-fold, and decreases levels of arachidonic acid by 10-fold.
This product is not for human or veterinary use，more warning is still in researching. Avoid direct contact with skin, eyes, respiratory tract, any discomfort, call your doctor right away.
IDFP (615250-02-7) Raw Powder
Min order 10grams.
The inquiry on normal quantity(Within 1kg) can be sent out in 12 hours after payment.
For larger order can be sent out in 3 working days after payment.
To be provided in the coming future.
Does IDFP Have Any Side Effects
The renal medulla, considered critical for the regulation of salt and water balance and long-term blood pressure control, is enriched in anandamide and two of its major metabolizing enzymes, cyclooxygenase-2 (COX-2) and fatty acid amide hydrolase (FAAH). Infusion of anandamide (15, 30, and 60 nmol·min-1·kg-1) into the renal medulla of C57BL/6J mice stimulated diuresis and salt excretion in a COX-2- but not COX-1-dependent manner. To determine whether endogenous endocannabinoids in the renal medulla can elicit similar effects, the effects of intramedullary isopropyl dodecyl fluorophosphate (IDFP), which inhibits the two major endocannabinoid hydrolases, were studied. IDFP treatment increased the urine formation rate and sodium excretion in a COX-2- but not COX-1-dependent manner. Neither anandamide nor IDFP affected the glomerular filtration rate. Neither systemic (0.625 mg·kg-1·30 min-1 iv) nor intramedullary (15 nmol·min-1·kg-1·30 min-1) IDFP pretreatment before intramedullary anandamide (15-30 nmol·min-1·kg-1) strictly blocked effects of anandamide, suggesting that hydrolysis of anandamide was not necessary for its diuretic effect. Intramedullary IDFP had no effect on renal blood flow but stimulated renal medullary blood flow. The effects of IDFP on urine flow rate and medullary blood flow were FAAH-dependent as demonstrated using FAAH knockout mice. Analysis of mouse urinary PGE2 concentrations by HPLC-electrospray ionization tandem mass spectrometry showed that IDFP treatment decreased urinary PGE2 These data are consistent with a role of FAAH and endogenous anandamide acting through a COX-2-dependent metabolite to regulate diuresis and salt excretion in the mousekidney.
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